Biotinylated anti his tag antibody
WebJan 1, 2010 · Our anti-His tag antibody portfolio has been tested in ELISA, flow cytometry, immunofluorescence and immunoprecipitation. Clone AD1.1.10 has been successfully used in 12 papers. Anti-His tag antibodies are available directly conjugated to HRP and AP - making the use of secondary antibodies in Western blotting redundant. WebGenScript His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody.The antibody recognizes fusion protein with His tag which is located at either C …
Biotinylated anti his tag antibody
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WebSino Biological is a global provider of “One-stop” CRO service to the pharmaceutical, biotechnology, diagnostic industries and academic research institutions. Learn More >. Antibody Fragment Production Services. … WebBiotinylated-His peptide to be used as a probe or control for Alpha no-wash assays.
WebHis Tag: Products. Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His … WebPVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Recombinant Monoclonal Antibody (Catalog # MAB050R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018 ). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under …
WebGenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THE™ His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity … WebFeb 12, 2024 · The anti-FLAG antibody was incubated with or without TurboID at 37 °C for 3 h in the presence of 1 mM biotin. (D) Binding activity of biotin-labeling antibodies. Immunoblotting was performed using biotinylated anti-AGIA antibody or anti-FLAG antibody as shown in (B) and (C). HRP-conjugated anti-biotin antibody was used as …
WebPolyclonal Antibody for studying His-Tag. Cited in 149 publications. Validated for Western Blotting, Immunoprecipitation. ... Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody ... Loading of …
WebBiotin is a small molecule which non-covalently binds to avidin and streptavidin with very high affinity. The affinity of the interaction makes biotin an excellent conjugate for detection when used in … iron man line artworkWeb31-1048-02. Clone Name. RM146. Product Data Sheet. Biotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146. Specificity. RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to … port orchard bay street floodingWebBiotin-labeled anti-His-tag antibodies are used to indirectly label proteins tagged with 6XHis. This can be advantageous when working with partially purified protein, or in … iron man live wallpaper for pc tech rifleWebApr 6, 2024 · Anti-His-tag 6xHis ANTIBODY Home / Tag Antibodies / His (3x, 6x) / Anti-His-tag 6xHis ANTIBODY His-tag 6xHis (clone HIS,H8 / EH158) AS11 1771 Clonality: monoclonal Host: Mouse Reactivity: His-tag DATA SHEET IN PDF Qty: 259 € Buy 2 items of this product for 187.00 €/item Buy 3 items of this product for 171.00 €/item Add … port orchard basketballWebThe antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. References for anti-Avi-Tag antibody (ABIN1574261) (hide) port orchard bayWebBiotinylation. In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely ... port orchard barber shopsWebHis-tags. Molecular Weight: 0.2–1.6 kDa. 6x-His tag is 0.8 kDa. Size: 2–10 histidine residues Tag location: C- or N- terminals, or internal. Affinity Resin: transition metal ions, usually Ni 2+ Applications: protein purification Strengths: Small size so lower possibility of affecting fusion protein functionality. Doesn’t cause inclusion bodies. Limitations: Can … iron man lively wallpaper for pc