WebT4 polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5′-hydroxyl terminus of DNA or RNA. It also can catalyze the exchange of 5′-terminal phosphate groups. In contrast to the wild type E. coli enzyme, this phage derivative lacks 3′-phosphatase activity.

全部产品分类_南京伟沃生物科技有限公司_丁香通

WebT4 Polynucleotide Kinase Part Numbers: M4101, M4103 Transfers the γ-Phosphate from ATP to the 5´ End of Nucleotides Can be inactivated by heating at 68°C for 10 minutes … WebA 3'-phosphatase minus version of T4 PNK OptiKinase is a recombinant version of T4 Polynucleotide Kinase (T4 PNK) that has been genetically engineered for improved 5'-phosphorylation of DNA. Like wild-type T4 PNK, OptiKinase catalyzes the transfer of the γ-phosphate of ATP to the 5' hydroxyl end of polynucleotides, including double-stranded and hardest wood floors for dogs

T4 Polynucleotide Kinase (10 U/µL) - Thermo Fisher Scientific

WebT4 Polynucleotide Kinase 2 µL ATP, 10 mM* 2 µL T4 Polynucleotide Kinase 1 µL (10 U) Water, nuclease-free (#R0581) to 20 µL Total volume 20 µL * Prepare 10 mM ATP solution by combining 10 µL of 100 mM ATP solution (#R0441) and 90 µL of Water, nuclease-free. 2. Mix thoroughly, spin briefly and incubate at 37 °C for 20 min. 3. WebPage 1 of 3 Product Name: T4 Polynucleotide Kinase (3' phosphatase minus) Catalog Number: M0236S Concentration: 10,000 U/ml Unit Definition: One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid insoluble [³³P] in 30 minutes at 37°C. Lot Number: 10027506 Expiration Date: 11/2024 Storage Temperature: -20°C WebJan 7, 2013 · T4 polynucleotide kinase-phosphatase (Pnkp) exemplifies a family of enzymes with 5'-kinase and 3'-phosphatase activities that function in nucleic acid repair. The polynucleotide 3'-phosphatase reaction is executed by the Pnkp C-terminal domain, which belongs to the DxDxT acylphosphatase superfamily. hardest wood flooring species